From Signal to Form: Nod Factor as a Morphogenetic Signal Molecule to Induce Symbiotic Responses in Legume Root Hairs

This thesis explored the role of nod factor (NF) in inducing symbiotic responses in legume root hairs, primarily using Medicago truncatula as a model. The research demonstrated that NF could induce root hair reorientation and gene expression with as little as a single NF molecule. The study also revealed that NF-induced root hair responses could be modulated by various pharmacological agents, indicating the involvement of calcium influx and heterotrimeric G-protein coupled phosphoinositide signaling in these processes. Additionally, the research highlighted differences in NF signaling pathways, with one branch leading to root hair curling and another to gene expression. The findings contributed to a deeper understanding of the molecular mechanisms underlying Rhizobium-legume symbiosis.