Multiplex Matrix Network Analysis of Protein Complexes in the Human TCR Signalosome

    August 2016 in “ Science Signaling ”
    Stephen Smith, Steven C. Neier, Brendan Reed, Tessa R. Davis, Jason P. Sinnwell, Jeanette E. Eckel‐Passow, Gabriel F. Sciallis, Carilyn N. Wieland, Rochelle R. Torgerson, Diana Gil, Claudia Neuhauser, Adam G. Schrum
    TLDR Alopecia areata patients show unique protein activity patterns, suggesting imbalanced signaling pathways.
    The study applied a multiplex matrix system to analyze protein complexes in the T cell receptor (TCR) signalosome, focusing on samples from Jurkat cells and 7 alopecia areata patients compared to 5 controls. It identified distinct PiSCES biosignatures in alopecia areata patients, characterized by enhanced basal TCR signaling network activity and a potential imbalance between GADS and GRB2 activity. This imbalance might influence pathways related to cytoskeletal remodeling and integrin signaling. The study demonstrated that PiSCES analysis could effectively distinguish between disease and control conditions, providing insights into the underlying signaling pathways involved in alopecia areata. Despite limitations in detecting all protein interactions, the approach offered valuable insights into protein network activities and potential disease pathways.
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