From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy

The study explored the potential of using an immortalized MSC line (HATMSC1) derived from human adipose tissue to produce cytokines for regenerative medicine or immunotherapy. The HATMSC1 cell line produced higher levels of biologically active factors compared to primary MSCs, with some factors being unique to the cell line. The conditioned medium from HATMSC1 enhanced cell proliferation, adhesion, and endothelial function. Hypoxic culture conditions increased the production of pro-angiogenic factors like VEGF and IL-8, while immunomodulatory factors such as GM-CSF and IL-6 were also elevated. These findings suggested that the conditioned media from HATMSC1 could be tailored for use in various medical conditions, including regenerative and inflammatory diseases.