Light Sheet Fluorescence Microscopy Quantifies Calcium Oscillations in Root Hairs of Arabidopsis Thaliana

The study demonstrated that light sheet fluorescence microscopy was an effective method for imaging and quantifying calcium oscillations in the root hairs of Arabidopsis thaliana. By using this technique, researchers were able to perform live imaging of root hairs in seedlings expressing the calcium indicator Yellow Cameleon 3.6, allowing them to measure the calcium gradient in numerous root hairs. The study characterized these calcium oscillations and correlated them with the rate of hair growth. Additionally, the method was utilized to screen the effects of auxin on the properties of growing root hairs.