Liposome-Based Carriers for CRISPR Genome Editing

The document reviews the use of liposomes as carriers for CRISPR genome editing technology. Liposomes, small vesicles composed of lipid bilayers, can encapsulate and protect CRISPR components, aiding their delivery into target cells. Various types of liposomes, including cationic lipid-based, hybrid, fusogenic, PEGylated, multifunctional, and stimuli-responsive liposomes, have been used for this purpose. The document highlights the potential of this technology in gene therapy, with examples of successful applications in preclinical models. However, challenges such as efficient delivery across different cell types, minimizing off-target effects, and regulatory approval remain. Strategies to overcome these include improved guide RNA design, base and prime editing, targeted liposome delivery, and controlled release mechanisms. Further research is needed to assess safety, efficacy, and long-term effects in human disease treatment.