Lights Triggered Differential Accumulation of Antioxidant and Antidiabetic Secondary Metabolites in Callus Culture of Eclipta Alba L.

The study investigated the effects of multispectral lights on the growth and phytochemical accumulation in callus cultures of Eclipta alba L. Red light exposure resulted in the highest dry weight (11.2 g/L) and significantly increased the content of phenolics and flavonoids, as well as major compounds like wedelolactone and demethylwedelolactone. Antioxidant, antidiabetic, and lipase inhibitory activities were also optimized under red light, highlighting its potential to enhance the therapeutic properties of E. alba. The findings suggested that using multispectral lights in in vitro cultures could effectively boost phytochemical production.