Author Response: Lhx2 as a Progenitor-Intrinsic Modulator of Sonic Hedgehog Signaling During Early Retinal Neurogenesis
October 2022
TLDR Lhx2 is a crucial regulator of the Sonic Hedgehog signaling in early mouse retinal development.
The document is a response to reviewers' comments on a study investigating the role of Lhx2, a transcription factor, in modulating Sonic Hedgehog (Shh) signaling during early retinal neurogenesis. The authors clarified their methods, provided additional control images, adjusted their figures, corrected errors, and added references. They addressed questions about their data, including their choice of specific timepoints, the variation in Gli1 expression levels, and changes in Cyclin D1 and Hes1 levels. They also explained their reasons for not performing certain analyses and discussed the challenges of their experiments. The authors provided evidence that Lhx2 mRNA is downregulated within 48 hours of tamoxifen treatment and acknowledged that further work is needed to understand the potential functional significance and mechanisms causing this effect.
