Kinetics of Titan Cells Generation and Transcriptome Modifications Comparing Three In Vitro Protocols
September 2022
in “
Medical Mycology
”
TLDR Three different methods were compared for creating Titan cells, a type of fungus cell. The OZ method made the most cells initially, but the number dropped quickly. The EB method also made a lot of cells, but the number also dropped. The AA method made fewer cells, but the number stayed steady. The methods also affected which genes were active in the cells.
The study compared three in vitro protocols for generating Titan cells (TC), a morphological change in Candida neoformans, a fungus that can cause infections in humans. The protocols were evaluated for the size and quantity of TC produced over a 72-hour period. The OZ protocol generated the highest percentage of TC, 63.1% and 58.2% at 18 and 24 hours respectively, but decreased to 6.7% at 48 hours. The EB protocol reached a high of 46.7% TC at 24 hours, then dropped below 10%. The AA protocol did not reach as high a quantity of TC, but it remained constant over 72 hours. RNA sequencing showed differences in gene expression at different time points. The study concluded that the kinetics of TC generation varied significantly between the protocols, with a significant variation in the transcriptome. This finding is important for future research into the genes associated with TC generation.
