Kinetics of Titan Cells Generation and Transcriptome Modifications Comparing Three In Vitro Protocols
September 2022
in “
Medical Mycology
”
Studysummary This study found that three protocols for generating Titan cells in vitro from Candida neoformans vary in their kinetics and transcriptomic profiles, pointing to differences which could impact further research.
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The study compared three in vitro protocols for generating Titan cells (TC), a morphological change in Candida neoformans, a fungus that can cause infections in humans. The protocols were evaluated for the size and quantity of TC produced over a 72-hour period. The OZ protocol generated the highest percentage of TC, 63.1% and 58.2% at 18 and 24 hours respectively, but decreased to 6.7% at 48 hours. The EB protocol reached a high of 46.7% TC at 24 hours, then dropped below 10%. The AA protocol did not reach as high a quantity of TC, but it remained constant over 72 hours. RNA sequencing showed differences in gene expression at different time points. The study concluded that the kinetics of TC generation varied significantly between the protocols, with a significant variation in the transcriptome. This finding is important for future research into the genes associated with TC generation.
