연구논문 : 기능성 화장품 소재개발을 위한 in vitro 평가법

The document discussed the development of in vitro evaluation methods for cosmeceutical ingredients, focusing on whitening, anti-wrinkle, and hair growth products. It highlighted the importance of safety, efficacy, stability, and user convenience in cosmetic products, particularly in Korea, where functional cosmetics have grown since the separation of cosmetic law from pharmaceutical law in 2000. The paper introduced various in vitro methods, such as testing collagen and MMP-1 for anti-wrinkle effects, and using tyrosinase activity and melanocyte cultures for whitening effects. For hair growth, dermal papilla cell culture and hair organ culture were commonly used. The document emphasized the need for developing methods with higher relevance to human biology and reliable biomarkers, as current methods have limitations.