Implantation study on the potential of murine epidermal stem cell differentiation

The study investigated the differentiation potential of murine epidermal stem cells (ESCs) when seeded in a biodegradable carrier and implanted into syngeneic mice. The ESCs were induced to differentiate in vitro, stained with Hoechst 33342, and then seeded into a polyglycolic acid net with collagen gel before implantation. Results showed that the ESCs remained viable for at least 3 weeks post-implantation and differentiated into structures resembling hair follicles, glands, and native dermis. No rejection or severe side effects were observed up to 10 weeks after implantation. The study concluded that these ESCs could be used to create dermal equivalents capable of developing dermal appendages.