Evaluation of Immunoreactivity of Normal Tissues from Dogs Using Monoclonal Antibody B72.3

Monoclonal antibody B72.3, along with CC49 and CC83, was tested for immunoreactivity in normal canine tissues. B72.3 showed selective staining in epithelial cells of the gastrointestinal and respiratory tracts, salivary glands, esophagus, epididymis, uterus, thymus, hair follicles, and apocrine glands of the anal sac, but not in most other normal tissues. CC49, unlike B72.3 and CC83, also stained the endothelium of capillaries and small vessels. The study concluded that these antibodies had specific immunoreactivity for certain types of normal canine epithelial cells, particularly those associated with mucin production.