In Vitro Keratin Expression of Hair Cells

    November 1992 in “ Journal of dermatology ”
    Yukio Kitano, Katsuyuki Saito, Eriko Okamoto, Yuri Okano, Noriko Tanigaki-Obana, Kei Ito, Toshio Tazawa, Masaaki Ito
    TLDR Hair cells grown in a lab showed specific hair proteins.
    The study conducted by Yukio Kitano and colleagues in 1992 focused on isolating human hair follicles from the scalp using dispase and collagenase treatment, followed by dispersion into a cell suspension with trypsin. These cells demonstrated good proliferation and could be subcultured 7 to 8 times. The culture medium used was MCDB 153 HAA, supplemented with amino acids, hydrocortisone, insulin, EGF, and bovine brain extract, with a Ca++ concentration of 0.1 mM. Immunohistochemical analysis confirmed that these cells expressed keratins specific to hair-forming cells.
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