Preparation and Properties of Human Hair Keratin Film Lacking Keratin-Associated Proteins

    January 2015 in “ Sen i Gakkaishi ”
    Toshihiro Fujii, Yumiko Ito
    TLDR The new keratin film without KAPs stains better and could help study keratin functions.
    The study developed a novel keratin film lacking keratin-associated proteins (KAPs) using a Pre-cast method, which allowed for the selective isolation of keratin from human hair. This KAPs-free keratin film was compared to conventional and high acetic acid keratin films containing KAPs. The KAPs-free film exhibited a beige and semitransparent appearance, with a reticular structure and small surface particles. It showed higher color deposits when stained with oxidative hair color reagents compared to conventional films. The formation of cysteic acid was more rapid in these films than in hair samples. The study suggested that this film formation technology could be useful for evaluating various functions of keratin and KAPs.
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