Green synthesis of silver nanoparticles (AgNPs) using of Laurus nobilis L. leaf extracts and evaluating its antiarthritic activity by in vitro protein denaturation and membrane stabilization assays

The study focused on the green synthesis of silver nanoparticles (AgNPs) using Laurus nobilis L. leaf extracts to evaluate their antiarthritic activity. The AgNPs were characterized by UV-Vis spectroscopy and dynamic light scattering, showing a peak at 396 nm and an average size of 78.1 nm. The antiarthritic activity was assessed through in vitro protein denaturation and RBC membrane stabilization assays. AgNPs demonstrated significant inhibition of protein denaturation and membrane stabilization, with maximum inhibition percentages of 53.47% and 62.43% at a concentration of 1000 µg, respectively. These results suggested that AgNPs from L. nobilis could be developed into novel antiarthritic drugs, offering a potential alternative to traditional medications with fewer side effects.