Engineered Building Blocks to Print Endogenous Tissue and Complex Organs In Vitro

The document discussed the development of engineered micromodules called micro tissue precursors (μTP) for creating endogenous 3D tissues and complex organs in vitro. These μTPs served as a new class of bio-ink for organ printing, influencing the morphology and maturation of the final printed tissue. The study explored the μTP's fusion capability, ECM maturation, and mechanical properties, as well as their ability to be printed in various shapes. Additionally, a tissue-on-chip model was proposed using μTPs in a microfluidic platform to study the effects of flow rate and biochemical factors on tissue development. The research also demonstrated the potential of μTPs to create lung stroma and support bronchial epithelium differentiation. Furthermore, a human skin model was developed to maintain exogenous hair viability, showing promise for future hair follicle structure modeling.