Effect of adipose-derived stem cell-conditioned medium on the proliferation and migration of B16 melanoma cells

The study investigated the anticancer effects of adipose-derived stem cell-conditioned medium (ASC-CM) on B16 melanoma cells both in vitro and in vivo. Results showed that ASC-CM significantly reduced the proliferation and migration of these cells, as evidenced by an MTT assay and a wound migration model, respectively, with statistical significance (P<0.01). Cell cycle analysis revealed an increase in cells in the G1 phase and a decrease in the S and G2/M phases. In vivo experiments with C57BL/6 mice demonstrated a significant reduction in tumor volume following intratumoral injections of ASC-CM. These findings suggested that ASC-CM had an anti-tumorigenic effect and could potentially aid in melanoma treatment.