Cutaneous Two-Stage Chemical Carcinogenesis

The document outlined a protocol for inducing skin cancer in mice using a two-stage chemical method involving the mutagen DMBA and the pro-inflammatory agent TPA. This approach enabled the study of tumor development, progression, and the underlying molecular and immunological pathways. It emphasized the significance of mouse strain susceptibility, genetic mutations, and chemical dosing in optimizing tumor induction, providing a framework for observing the progression from benign papillomas to squamous cell carcinoma in a controlled environment.