Culture of dispersed hair follicle cells from plucked out hairs without a feeder layer.

Dispersed hair follicle cells from plucked hairs were successfully cultured on collagen type IV without the need for a biological feeder layer. The study determined that the optimal calcium concentration for these cultures was 0.3-0.5 mM, which facilitated the best colony formation and growth, resulting in large colonies with a paving stone-like cell arrangement. The researchers proposed using these cultured hair follicle cells as an alternative to cultured epidermal keratinocytes for treating missing skin in wound dressings.