Diagnostic Applications and Therapeutic Options of Cascade CRISPR/Cas in the Modulation of miRNA in Diverse Cancers: Promises and Obstacles

The document reviews the potential of CRISPR/Cas systems, particularly CRISPR/Cas9, in modulating microRNAs (miRNAs) for cancer diagnostics and therapy. It highlights the promise of CRISPR/Cas technology in precise gene editing and miRNA detection, which are crucial for understanding and treating various cancers. miRNAs, such as miR-21 and miR-155, play significant roles in cancer progression and prognosis, making them valuable targets for therapy. The document discusses various delivery methods for CRISPR components, including viral vectors and nanoparticles, and emphasizes the need for precise delivery to maximize therapeutic efficacy and minimize off-target effects. Despite advancements, challenges remain in improving targeting specificity and reducing off-target mutations. The review also notes the potential of circRNAs in miRNA regulation and the importance of miRNAs in cancer and cardiovascular diseases, suggesting that while CRISPR/Cas systems hold promise for cancer treatment, further research is needed to address delivery and specificity issues.