Current And Prospective Strategies For Advancing The Targeted Delivery Of CRISPR/Cas System Via Extracellular Vesicles

The document reviews the potential of extracellular vesicles (EVs) as delivery vectors for the CRISPR/Cas system, emphasizing their advantages over traditional methods due to high biocompatibility and minimal immune reactions. It discusses strategies to enhance EV loading capacity, safety, and targeting, including modifications and artificial EVs. Despite their promise, challenges such as optimizing delivery efficiency and addressing loading issues remain. The review suggests that engineered EVs could significantly improve CRISPR/Cas delivery, offering a safer and more effective alternative for gene editing, with ongoing advancements potentially revolutionizing therapeutic genome editing.