The 532 Nm Laser Treatment Promotes the Proliferation of Tendon-Derived Stem Cells and Up-Regulates Nr4a1 to Stimulate Tenogenic Differentiation

November 2021 in “ Research Square (Research Square) ”

Ming Li, Yiping Zhu, Qing Pei, Yuping Deng, Ni Tien

low-level laser therapy LLLT 532 nm laser tendon-derived stem cells TDSCs Nr4a1 tenogenic differentiation

TLDR A 532 nm laser at 15 J/cm2 speeds up tendon healing by increasing tendon stem cell growth and tendon-related gene activity.

The study investigated the effect of low-level laser therapy (LLLT) with a wavelength of 532 nm on the proliferation and differentiation of tendon-derived stem cells (TDSCs) from Sprague-Dawley rats. The ideal energy was determined to be 15 J/cm2. The results showed that at this energy density, the proliferation capacity of TDSCs improved (2.73 ± 0.24 vs. 1.81 ± 0.71, P < 0.05), and the expression of genes related to tenogenic differentiation of TDSCs significantly increased (P < 0.01), indicating potential for tenogenic differentiation. The study also found that Nr4a1, a gene identified through RNA-seq and bioinformatics analyses, was involved in the tenogenic differentiation process of TDSCs regulated by the 532 nm laser treatment. The study concluded that a 532 nm laser with 15 J/cm2 could accelerate tendon healing by regulating the process of TDSC proliferation and tenogenic differentiation through up-regulating Nr4a1.

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