Effects of 2-Ethylhexanoic Acid on Reproduction and Postnatal Development in Wistar Rats

The study investigated the reproductive toxicity of 2-ethylhexanoic acid (2-EHA) in Wistar rats, with 24 animals per sex per group receiving doses of 100, 300, or 600 mg/kg. Results showed a dose-dependent decrease in fertility, with increased time to mating and total infertility at higher doses. Male rats exhibited decreased sperm quality, with reduced motility and increased abnormal sperm at higher doses. The average litter size decreased by 16% at 600 mg/kg, and while birth weights were unaffected, body weight gain during lactation was slower. Pups showed delayed physical development and abnormal features at higher doses. A single 600 mg/kg dose given to pregnant females reduced implantations and caused resorptions. The study concluded that 2-EHA impaired fertility and delayed postnatal development, likely due to disturbed implantation and teratogenic effects.